Journal:

Article Title: Genome Diversification in Phylogenetic Lineages I and II of Listeria monocytogenes : Identification of Segments Unique to Lineage II Populations †

doi: 10.1128/JB.185.18.5573-5584.2003

Figure Lengend Snippet: Strains used in this study

Article Snippet: To compare genome diversity, independent 2-μg aliquots of strain 10403s reference DNA and DNA from a given test strain were random primed using Cy-5 and Cy-3 dye-labeled nucleotides, respectively, with BioPrime DNA labeling kits (Life Technologies, Rockville, Md.).

Techniques:

Journal:

Article Title: Genome Diversification in Phylogenetic Lineages I and II of Listeria monocytogenes : Identification of Segments Unique to Lineage II Populations †

doi: 10.1128/JB.185.18.5573-5584.2003

Figure Lengend Snippet: PCR analysis of the lmo0421-lmo0423 region. Agarose gel electrophoresis of PCR amplification products derived from PCR amplification of the lmo0420-lmo0424 region. Products from lineage I and lineage II strains are indicated. The control strain, lineage II strain 10403s, is indicated on the right end of the gel along with the 3- and 1-kb markers from the size standards. The map underneath depicts the region in lineage II and lineage I strains. The lineage I map is based on sequence analysis of PCR amplification products from two independent lineage I strains.

Article Snippet: To compare genome diversity, independent 2-μg aliquots of strain 10403s reference DNA and DNA from a given test strain were random primed using Cy-5 and Cy-3 dye-labeled nucleotides, respectively, with BioPrime DNA labeling kits (Life Technologies, Rockville, Md.).

Techniques: Agarose Gel Electrophoresis, Amplification, Derivative Assay, Sequencing